Experimental Procedure Chill the rubbing alcohol in the freezer. You will need it later.
I mixed 5 ml of detergent with. I added 40 ml of distilled water to the detergent solution, and I stirred the solution. I then broke down the strawberry cells into basic components: I added 10 ml of the extraction buffer solution to a plastic bag.
I removed the leaves and sepals from the strawberry and placed it inside the Dna strawberry conclusion. I removed the air from the bag, and I compressed the strawberry.
I crushed the bag against the table and formed a strawberry slurry. I then extracted the strawberry DNA from other large solids in the slurry: I placed a two inch square of cheesecloth over a funnel and secured it with my fingers.
I poured the slurry over the cheesecloth, which separated the large solids; and I collected the liquid and DNA in the test tube. I discarded the strawberry solids that remained in the cheesecloth.
Finally, I isolated the DNA from the liquid in the test tube: I filled a quarter of the test tube with strawberry liquid, and I added the same amount of ice cold ethanol.
I placed a long wooden splint into the test tube, and I stirred it without shaking the tube. The DNA became visible in the ethanol layer at the top of the test tubes. The detergent degrades the phospholipid bilayer of the cell and nuclear membranes after the strawberry is crushed.
At this step of the experiment, the detergent dissolves cell and nuclear membranes, and DNA remains in the extraction buffer solution.
The strawberry liquid, which contains DNA, is in the container on the bottom. The ethanol is the top layer, and it contains visible strands of DNA. The DNA is visible because it is not soluble in a nonpolar solvent such as ethanol.
The extraction of DNA was facilitated by the presence of eight copies of each chromosome, or an octoploid genome, in the strawberry.
In contrast, humans have a diploid genome, which includes only two copies of each chromosome. I degraded the strawberry cell walls, cell membranes, and nuclear membranes by crushing the berry and mixing it with a buffer of saltwater and dish detergent.
The mashing physically disrupted the cell walls, the detergent dissolved the membrane phospholipid bilayers, and the salt provided optimum osmolarity. The naturally occurring pectinase and cellulase enzymes also promoted cell wall degradation.
The large solids were then separated by filtration, and the resulting solution was collected in a test tube. At that time, the DNA was dissolved in water, which is a polar solvent; and sodium ions bonded with the negatively charged DNA via an ionic bond.
After the addition of ethanol, DNA precipitated into the nonpolar layer, which made the long DNA strands easily visible to the naked eye. If I were to repeat this experiment, I would attempt to extract DNA from other fruits; including other berries, apples, and bananas.
I anticipate that it would be more difficult to extract DNA from cells that have fewer copies of chromosomes.
Finally, I would vary the amount and timing of salt added to the extraction buffer. I anticipate that the quantity of DNA seen in the alcohol layer would vary if salt were omitted, as the DNA would be less polar at the time of extraction.
I would also be interested to learn if adding more salt would result in the extraction of a larger quantity of DNA.A super fun experiment we found on KidScienceApp is extracting DNA from strawberries.
DNA is the “blueprint” for all organisms. It can be found in the nucleus of the cell. To get to the DNA, the cell wall needs to be broken down and the DNA needs to be separated from the rest of the cell. Extracting dna from strawberries lab conclusion essay.
Extracting dna from strawberries lab conclusion essay. 4 stars based on reviews monstermanfilm.com Essay. Bonnie and clyde film analysis essay essay on 20 unsolved crime. Strawberry DNA Conclusion The purpose of this lab was to extract DNA from strawberries.
When we put rubbing alcohol into the mixture of extracted strawberry juice, dish soap, and . Feb 16, · Introduction: Extracting DNA From Strawberries By jmjohnson Follow This is a great introductory activity while introducing the subject of DNA and cellular structure.
Analysis & Conclusion/Discussion Drafting Analysis: My averages of all three fruits range from a difference compared to the other fruit of , and 2. The total number of DNA for each fruit had a big variation between strawberries compared to kiwis and bananas with a difference of 5 and 6.
Strawberry DNA was long, clear coloured and. If the lab is conducted properly then we should be able to view a visible amount of DNA from the strawberry and detergent mixture.
Variables: The independent variable in this experiment is the strawberry mixture while the dependent variable is the amount of DNA extracted.